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Research focus
Biochemistry · Biocatalysis

Focus

My scientific work sits at the intersection of biochemistry and computation. I've spent years studying how enzymes behave when attached to solid supports — a deceptively hard problem that determines whether a biocatalytic process is economically viable or not. That passion led me to start inseit.

At inseit, we're building an AI-powered platform that predicts and optimises how enzymes are immobilised on support materials for industrial applications. We work with companies in pharma, food, and environmental chemistry who need faster, more reliable enzyme development pipelines. Backed by University of Bern, Venture Kick, and BRIDGE, among others.

Publications (32)
ORCID ↗
32
Co-author 2025

Immobilization of Thrombin on Agarose-Based Supports for Affinity Tag Removal

Almada, J.C.; Marco-Martin, M.; Roura Padrosa, D.; Velasco-Lozano, S.. Biomacromolecules. DOI ↗

31
Co-author 2025

Crystallization-Assisted Asymmetric Synthesis of Enantiopure Amines Using Membrane-Immobilized Transaminase

Meersseman Arango, H.; Bachus, N.; Nguyen, X.D.L.; Bredun, B.; Luis, P.; Leyssens, T.; Roura Padrosa, D.; Paradisi, F.; Debecker, D.P.. Chem & Bio Engineering. DOI ↗

30
Co-corresp. 2025

Biocatalysis in packed-bed reactors: immobilization as an enabling technology

Fernández Regueiro, C.L.; Roura Padrosa, D.; Paradisi, F.. Comptes Rendus. Chimie. DOI ↗

29
Co-author 2025

Biocatalytic Reduction of Heterocyclic Imines in Continuous Flow with Immobilized Enzymes

Benítez-Mateos, A.I.; Lim, D.; Roura Padrosa, D.; Marchini, V.; Wu, H.; Buono, F.; Paradisi, F.. ACS Sustainable Chemistry & Engineering. DOI ↗

28
Co-author 2025

A novel ene-reductase from Halomonas elongata for flow biocatalytic synthesis

Pillet, L.; Fernández Regueiro, C.L.; Busch, M.R.; Roura Padrosa, D.; Paradisi, F.. RSC Advances. DOI ↗

27
Co-author 2024

Microtiter Plate Immobilization Screening for Prototyping Heterogeneous Enzyme Cascades

López, I.L.; Sánchez-Costa, M.; Orrego, A.H.; Zeballos, N.; Roura Padrosa, D.; López-Gallego, F.. Angewandte Chemie. DOI ↗

26
Co-author 2024

Solvent Switching in Continuous Multistep Chemoenzymatic Synthesis

Díaz-Kruik, P.; Roura Padrosa, D.; Hegarty, E.; Lehmann, H.; Snajdrova, R.; Paradisi, F.. Organic Process Research & Development. DOI ↗

25
First author 2024

Biocatalyst Immobilization and Process Intensification: Production of (S)-Piperazine-2-carboxylic Acid

Roura Padrosa, D.; Wetzl, D.; Hildbrand, S.; Tosatti, P.; Sedelmeier, J.; Puentener, K.; Iding, H.; Paradisi, F.. Organic Process Research & Development. DOI ↗

24
Co-first 2024

Flow Biocatalysis for Chiral Molecules Synthesis

Roura Padrosa, D.; Benitez-Mateos, Ana I.; Paradisi, F.. Comprehensive Chirality. DOI ↗

23
Co-author 2024

Membrane-immobilized transaminases for the synthesis of enantiopure amines

Meersseman Arango, H.; Nguyen, X.D.L.; Luis, P.; Leyssens, T.; Roura Padrosa, D.; Paradisi, F.; Debecker, D.P.. RSC Sustainability. DOI ↗

22
Co-author 2023

Optimising Electrical Interfacing between the Trimeric Copper Nitrite Reductase and Carbon Nanotubes

Contaldo, U.; Padrosa, D.R.; Jamet, Hélèn.; Albrecht, M.; Paradisi, F.; Le Goff, A.. Chemistry – A European Journal. DOI ↗

21
First author 2023

Bioinformatic Analysis of Immobilized Enzymes: Towards a Better Understanding

Roura Padrosa, D.; Paradisi, F.. ChemBioChem. DOI ↗

20
First author 2023

Sustainable synthesis of L-phenylalanine derivatives in continuous flow by

Roura Padrosa, D.; Lehmann, H.; Snajdrova, R.; Paradisi, F.. Frontiers in Catalysis. DOI ↗

Structures
19
Co-author 2022

Back to the future: taking enzymes to the next level of sustainability

Padrosa, D.R.; Benítez-Mateos, A.I.; Paradisi, F.. The Biochemist. DOI ↗

18
Co-first 2022

Multistep enzyme cascades as a route towards green and sustainable pharmaceutical syntheses

Benítez-Mateos, A.I.; Roura Padrosa, D.; Paradisi, F.. Nature Chemistry. DOI ↗

17
First author 2022

How can Enzymes Help us With Manufacturing?

Roura Padrosa, D.; Paradisi, F.. Frontiers for Young Minds. DOI ↗

16
Co-author 2022

A novel thymidine phosphorylase to synthesize (halogenated) anticancer and antiviral nucleoside drugs

Benítez-Mateos, A.I.; Klein, C.; Roura Padrosa, D.; Paradisi, F.. Catalysis Science & Technology. DOI ↗

15
Co-corresp. 2022

Combined chemoenzymatic strategy for sustainable continuous synthesis of hordenine

Gianolio, S.; Roura Padrosa, D.; Paradisi, F.. Green Chemistry. DOI ↗

14
Co-author 2021

Fusion of Glutamate Dehydrogenase and Formate Dehydrogenase Yields a Bifunctional Efficient Biocatalyst for the Continuous Removal of Ammonia

Marchini, V.; Benítez-Mateos, A.I.; Roura Padrosa, D.; Paradisi, F.. Frontiers in Catalysis. DOI ↗

13
First author 2021

Efficient Amino Donor Recycling in Amination Reactions

Roura Padrosa, D.; Nisar, Z.; Paradisi, F.. Catalysts. DOI ↗

Structures
12
Corresponding 2021

CapiPy: python-based GUI-application to assist in protein immobilization

Roura Padrosa, D.; Marchini, V.; Paradisi, F.. Bioinformatics. DOI ↗

11
Co-first 2021

Flow biocatalysis 101: design, development and applications

Benítez-Mateos, A.I.; Contente, M.L.; Roura Padrosa, D.; Paradisi, F.. Reaction Chemistry & Engineering. DOI ↗

10
Co-author 2021

Minimalistic peptidic scaffolds harbouring an artificial carbene-containing amino acid

Lenzen, K.; Planchestainer, M.; Feller, I.; Roura Padrosa, D.; Paradisi, F.; Albrecht, M.. Chemical Communications. DOI ↗

09
First author 2021

Multi-gram preparation of cinnamoyl tryptamines as skin whitening agents

Roura Padrosa, D.; Contente, M.L.. Tetrahedron Letters. DOI ↗

Structures
08
Co-author 2020

A strategic Ser/Cys exchange in the catalytic triad unlocks acyltransferase-mediated synthesis

Contente, M.L.; Roura Padrosa, D.; Molinari, F.; Paradisi, F.. Nature Catalysis. DOI ↗

07
Co-author 2020

Uncommon overoxidative catalytic activity in a new halo-tolerant alcohol dehydrogenase

Contente, M.L.; Fiore, N.; Cannazza, P.; Roura Padrosa, D.; Molinari, F.; Gourlay, L.; Paradisi, F.. ChemCatChem. DOI ↗

06
Corresponding 2020

CapiPy: Python Based GUI-Application to Assist in Protein Immobilization

Roura Padrosa, D.; Marchini, V.; Paradisi, F.. Bioinformatics. DOI ↗

05
First author 2020

Cell-free biocatalytic syntheses of L-pipecolic acid

Roura Padrosa, D.; Benítez-Mateos, A.I.; Calvey, L.; Paradisi, F.. Green Chemistry. DOI ↗

Structures
04
First author 2019

Enhancing PLP-Binding Capacity of Class-III ω-Transaminase

Roura Padrosa, D.; Alaux, R.; Smith, P.; Dreveny, I.; López-Gallego, F.; Paradisi, F.. Frontiers in Bioengineering and Biotechnology. DOI ↗

Structures
03
First author 2019

Overcoming Water Insolubility in Flow: Enantioselective Hydrolysis of Naproxen Ester

Roura Padrosa, D.; De Vitis, V.; Contente, M.L.; Molinari, F.; Paradisi, F.. Catalysts. DOI ↗

02
First author 2018

Construction of Highly Stable Cytotoxic Nuclear-Directed Ribonucleases

Roura Padrosa, D.; Castro, J.; Romero-Casañas, A.; Ribó, M.; Vilanova, M.; Benito, A.. Molecules. DOI ↗

01
Co-author 2018

Genetically Fused T4L Acts as a Shield in Covalent Enzyme Immobilisation Enhancing the Rescued Activity

Planchestainer, M.; Roura Padrosa, D.; Contente, M.; Paradisi, F.. Catalysts. DOI ↗